ISOLATION AND CHARACTERIZATION OF AMYLASE-PRODUCING MICROBES FROM THE DIGESTIVE TRACT OF TILAPIA

Sri Wahyuni; Teguh Satria Amin; Nomi Noviani; Leni Handayani

doi:10.51612/teunuleh.v2i4.68

Authors

Sri Wahyuni Universitas Muslim Nusantara Al Washliyah
Teguh Satria Amin Universitas Muslim Nusantara Al Washliyah
Nomi Noviani Universitas Muslim Nusantara Al Washliyah
Leni Handayani Universitas Muslim Nusantara Al Washliyah

DOI:

https://doi.org/10.51612/teunuleh.v2i4.68

Abstract

Enzymes are natural products that play an important role in various industrial applications, from food processing to chemical products. One of the most widely used enzymes is amylase. Amylase is an enzyme that plays a role in starch hydrolysis. Amylolytic bacteria can be isolated in the digestive tract of fish. This is most bacteria that occupy the digestive tract have high activity. This study aims to obtain a potential isolate producing amylase bacteria whose activity can be determined through morphological characterization and activity test of enzyme-producing bacteria. The results obtained from this study were ten isolates with the potential to produce amylase enzymes, which were marked with a positive test after the addition of iodine.

Keywords:

Enzymes, Amylase, Tilapia Fish, Isolation

Downloads

Download data is not yet available.

References

Qonita, S. B., Johan, V. S., & Rahmayuni, R. (2018). Identifikasi Bakteri Asam Laktat dari Nira Aren Terfermentasi Spontan. Jurnal Online Mahasiswa Fakultas Pertanian, 5(1), 1-12.

Rahmiati, Pujianto, S., & Kusdiyantini, E. (2016). Eksplorasi Mikroba Penghasil Enzim-Enzim Hidrolitik di Kawasan Taman Nasional Lore Lindu Sulawesi Tengah. Jurnal Bioma: Berkala Ilmiah Biologi, 18(1), 14-19. doi:10.14710/bioma.18.2.14-19

Reddy, N., Nimmagadda, A., & Rao, K. R. (2003). An Overview of the Microbial ?-Amylase Family. African Journal of Biotechnology, 2(12), 645-648. doi:10.5897/AJB2003.000-1119

Sunaryanto, R., & Kaseno. (2004). Pemisahan Enzim Glukoamilase dari KalduFermentasi Menggunakan Membran Ultrafiltasi. Prosiding Seminar Nasional Rekayasa Kimia dan Proses, F(9), (9)1-5.

Syamsudin, Purwati, S., & Rizaludin, A. T. (2008). Efektivitas Enzim dalam Sistem Lumpur Aktif Pada Pengolahan Air Limbah Pulp dan Kertas. Jurnal Selulola, 43(2), 83-92. doi:10.25269/jsel.v43i02.156

Tjahjaningsih, W., Masithah, E. D., Pramono, H., & Suciati, P. (2016). Aktivitas Enzimatis Isolat Bakteri Asam Laktat Dari Saluran Pencernaan Kepiting Bakau (Scylla Spp.) Sebagai Kandidat Probiotik. Jurnal Ilmiah Perikanan dan Kelautan, 94-108. doi:10.20473/jipk.v8i2.11182

Vuyst, L. D., & Vandamme, E. J. (1994). Antimicrobial Potential of Lactic Acid Bacteria. In Bacteriocins of Lactic Acid Bacteria (pp. 91-142). Springer, Boston, MA. doi:10.1007/978-1-4615-2668-1_3